Journal: Scientific Reports

Article Title: Modeling ErbB2-p130Cas interaction to design new potential anticancer agents

doi: 10.1038/s41598-019-39510-w

Figure Lengend Snippet: Impact of 1 and 2 compounds on BT474 breast cancer cells. ( A ) To assess whether 1 and 2 could trigger cytotoxic effects on living cells, BT474 cells were treated with serial dilutions of 1 and 2 (or sterile DMSO). After three days, live cells were detected by standard MTT assay, highlighting the appearance of cytotoxic effects above 75 μM for compound 1 . Compound 2 did not show cytotoxic effects even at 250 μM. ***p < 0.001; ****p < 0.0001. ( B ) 1 and 2 compounds inhibit cell proliferation of BT474 and ( C ) SKBR3 breast cancer cell lines. Cells were treated with different concentration of the 1 and 2 and proliferation was assayed three days later by performing MTT assay. Results were fitted with four-parameter dose-response curve using GraphPad Prism7 software. ( D ) EC50 values for 1 and 2 in BT474 and SKBR3 breast cancer cell lines are represented as histogram with 95% confidence intervals. ( E ) SKBR3 cells were treated with Trastuzumab (25 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01. ( D ) SKH100 Trastuzumab-resistant cells were treated with Trastuzumab (100 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01.

Article Snippet: Results were fitted with four-parameter dose-response curve using GraphPad Prism7 software. ( D ) EC50 values for 1 and 2 in BT474 and SKBR3 breast cancer cell lines are represented as histogram with 95% confidence intervals. ( E ) SKBR3 cells were treated with Trastuzumab (25 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses.

Techniques: MTT Assay, Concentration Assay, Software

Journal: Toxins

Article Title: Combined Cytotoxicity of the Phycotoxin Okadaic Acid and Mycotoxins on Intestinal and Neuroblastoma Human Cell Models

doi: 10.3390/toxins10120526

Figure Lengend Snippet: Summary of cytotoxicity of typical A. sydowii metabolites, dust storm/shellfish-associated mycotoxins, okadaic acid algal toxins on HT-29 and SH-SY5Y cells after 24 h exposure. Inhibitory concentration 50% (IC 50 ) values and 95% confidence interval (CI) were calculated from four replicates using the four-parameter logistic model (4PL) model.

Article Snippet: Briefly, the dose response curves were fitted with the four-parameter logistic model (4PL), and 95% asymptotic confidence intervals were calculated using GraphPad Prism 7.

Techniques: Concentration Assay